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Video Script :-

Blood Agar Media

Blood agar is an enriched media , which capable of growing a range of microorganisms of clinical significance.
Fastidious organisms, such as streptococci, do not grow well on ordinary growth media.
BUT Blood agar is a type of growth medium that encourages the growth of bacteria, such as streptococci, that otherwise wouldn’t grow.
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Also Blood contains inhibitors , for certain bacteria such as Neisseria and Haemophilus genera and the blood agar must be heated to inactivate these inhibitors and to release essential growth factors such as x factor & V factor .
But When Heating of blood agar converts it into chocolate agar and supports the growth of these bacteria.

Blood agar can be made selective for certain pathogens , by the addition of antibiotics, chemicals or dyes. Examples includes crystal violet blood agar to select Streptococcus pyogens from throat swabs, and kanamycin or neomycin blood agar , to select anaerobes from pus.

Composition Of Blood Agar

Blood agar media consist of ,
Pancreatic digest of casein
Papaic digest of soy meal
0.5% NaCl
Agar
Distilled water &
5% Sheep Blood

Combine of the ingredients and final pH should be 7.3
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Uses Of Blood Agar Media

There 3 main uses of Blood agar , they are
Isolation organisms
Identification &
Detect antimicrobial susceptibility.

After spreading specimen on blood agar media , can be examine isolated pathogen organisms colonies on blood agar media. So blood agar really important to Isolate medically significant organisms.
Also blood agar media gives good colony appearance , hemolysis patterns such as Alpha , Beta & gama & pigment production.

Quality control of Blood Agar

Using Quality control of blood agar , we can identify whether our media in good quality or not , If not we have to discard all media

There several method to Quality control Blood agar ,
The pH of the blood agar range should from 7.2 to 7.6 at room temperature.
Incubate the plates in a carbon dioxide enriched atmosphere at 35-37°C overnight & check there any contamination present or not
Check the growth of positive control organisms & see colony , morphology ,
In this case these positive control organisms must grow on blood agar media & gives identical colony morphology , So
Staphylococcus aureus – should give good growth with white coloured colonies
Streptococcus pyogenes – should give good growth with pale colonies and beta haemolysis
And Streptococcus pneumonia – should give good growth with gray coloured colonies & alpha hemolysis

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